Research and Application of UV Visible Spectrophotometer

The use of microelectronics and computer technology has enabled the automation of analytical instruments. The development of analytical instruments mainly presents the following features:
(1) On-line analysis, real-time detection, and more and more portable instruments.
(2) The development of combined instrumentation technology makes it possible to complete more complex analytical tasks.
(3) High-throughput analysis requires the analysis and testing of a large number of samples per unit time.

Spectral analysis instruments are the most widely used and also the best analytical instrument for localization. Atomic absorption spectrometers, atomic fluorescence spectrometers, and atomic emission spectrometers are the most commonly used atomic spectrometers. Molecular spectrometers commonly used include ultraviolet-visible spectrophotometers, near-infrared spectroscopy, and Raman spectroscopy.

The Lambda1050N950 series UV-Vis spectrophotometer developed by PerkinElmer of the United States, and the Cary6000i UV-Visible near-infrared spectrophotometer of Agilent Inc. represent the world-leading level of such instruments. China's Beijing General Analysis TU-1950 series UV-Vis spectrophotometer and Beijing Beifen Rayleigh UV-2100N2200 UV-Vis spectrophotometer have higher technical level in the domestic market, but there is still a certain gap with similar foreign products.

The components or components that can be used for detection in the analysis of agricultural products and foods by the UV-Vis spectrophotometer are protein, lysine, glucose, vitamin C, nitrate, nitrite, arsenic, mercury, etc.; can be used in plant biochemical analysis. Detection of chlorophyll, total nitrogen and enzyme activity, etc.; can be used to detect niacin, gossypol, phosphine and methyl esters in feed assays. Wu Suxi et al., according to nicotine (commonly known as nicotine), react with methyl orange in a pH 5.0 environment to form a colored complex. The concentration of the complex in chloroform and its absorbance at visible wavelengths Based on the principle of proportionality, a spectrophotometer was used to determine the content of nicotine in waste tobacco extract. Cui Qin et al used a spectrophotometer to determine the content of chlorophyll in dehydrated leaves of wheat. The results showed that the chlorophyll content of leaves in both dry and fresh state was not affected without greening.

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